# Title: 19062019-Byk832 (ETH strain) transformation with pbK549 (very low effciency) 😔 😕

# Date:

19062019

# Objective:

This transofrmation is in order to insert the plasmid expressing the transposase and transposon in Byk832, to make a 1st round of the SATAY experiment. This strain BYK832 is: ade2::NAT ura3- and the plasmid is 👇 :

The digestion with EcoRV, to confirm the right plasmid from the genomic prep done on the bacteria expression pbk549, was succesful 😃 Look here 👇

# Method

Yeast transformation protocol

# DNA concentration

10ul of 600 ng/ul plasmid pbk549 = 6 ug

# Selection plates

• -URA plates for the transformants and negative control of the non transformants

# Results

• Around 24 colonies did growth... No so great efficiency.
• No growth in the negative control

# Conclusion