# Date

24092019-26092019

# Objective

To get a diploid that I am able to select for with marker selection.

# Method

• 23092019: Incubation of single colonies and not multiple colonies (from the transformation plate) of ylic134 in CSM-URA , and yEk7a from glycerol stock.
• 24092019: Clear tubes 😦 , no growth of the colonies taken of ylic134 (maybe it is because ylic133_3 still grows very slowly)
  • I took more colonies from the transformation plate and transfer them grow in CSM-URA
  • I did replica plating of the transformation plate onto a another -ura plate , to check the viability of the transformants on -ura.

# Results

• The transformation with pLL112 plasmid did not work.
• No colonies did grow in the replica plate in -URA

# Conclusion

• The mating never could happened because I did not get any ylic133+pLL112 cells 😦