# Title : 17062020 Experimental Plan to accomplish 2020 experimental goals.

# Date

17062020

# Objective

• To elaborate a realistic plan to continue doing experiments.

# Method

• Look at the agreed plan from the yearly meeting.
• Draw scenarios/ possibilities due to currently crisis of covid-19.

# Agreed plan

1. Measure the essential genes in each of the genetic backgrounds of the trajectory.

# Scenarios to achieve that plan

• Scenario 1: Ideal

  • Overlap with Enzo and Wessel experiments
    • Enzo and Wessel do the SATAY on $\Delta bem1$ and $\Delta bem1\Delta bem3$
    • Enzo will do the SATAY of $\Delta bem1\Delta bem3\Delta nrp1\Delta bem2$
  • I will do the SATAY and strain construction of $\Delta bem1\Delta bem3\Delta nrp1$

• Scenario 2: Probable

  • Overlap with Enzo and Wessel experiments
    • Enzo and Wessel do the SATAY on $\Delta bem1$.
    • Enzo will do the SATAY of $\Delta bem1\Delta bem3\Delta nrp1\Delta bem2$
    • I will do the SATAY and strain construction of $\Delta bem1\Delta bem3\Delta nrp1$ and for $\Delta bem1\Delta bem3$.

# Consequences for each scenario

• Scenario Ideal :

  • Develop a plan for the strain construction (knockout the ade2 gene in ylic128)(~ 2 months). It can start in September 2020.
  • Do the SATAY on all strains (~ ? months)

• Scenario Probable :

  • Develop a plan to have $\Delta bem1\Delta bem3 $ ura3- ade2-.(~ 3 months) It can start in September 2020.
  • Develop a plan for the strain construction (knockout the ade2 gene in ylic128)(~ 2 months). It can start in September 2020.
  • Do SATAY on all strains.

# Conclusion