# Title : 17062020 Experimental Plan to accomplish 2020 experimental goals.
# Date
17062020
# Objective
- To elaborate a realistic plan to continue doing experiments.
# Method
- Look at the agreed plan from the yearly meeting.
- Draw scenarios/ possibilities due to currently crisis of covid-19.
# Agreed plan
- Measure the essential genes in each of the genetic backgrounds of the trajectory.
# Scenarios to achieve that plan
Scenario 1: Ideal
- Overlap with Enzo and Wessel experiments
- Enzo and Wessel do the SATAY on $\Delta bem1$ and $\Delta bem1\Delta bem3$
- Enzo will do the SATAY of $\Delta bem1\Delta bem3\Delta nrp1\Delta bem2$
- I will do the SATAY and strain construction of $\Delta bem1\Delta bem3\Delta nrp1$
- Overlap with Enzo and Wessel experiments
Scenario 2: Probable
- Overlap with Enzo and Wessel experiments
- Enzo and Wessel do the SATAY on $\Delta bem1$.
- Enzo will do the SATAY of $\Delta bem1\Delta bem3\Delta nrp1\Delta bem2$
- I will do the SATAY and strain construction of $\Delta bem1\Delta bem3\Delta nrp1$ and for $\Delta bem1\Delta bem3$.
- Overlap with Enzo and Wessel experiments
# Consequences for each scenario
Scenario Ideal :
- Develop a plan for the strain construction (knockout the ade2 gene in ylic128)(~ 2 months). It can start in September 2020.
- Do the SATAY on all strains (~ ? months)
Scenario Probable :
- Develop a plan to have $\Delta bem1\Delta bem3 $ ura3- ade2-.(~ 3 months) It can start in September 2020.
- Develop a plan for the strain construction (knockout the ade2 gene in ylic128)(~ 2 months). It can start in September 2020.
- Do SATAY on all strains.