# Title : 17062020 Experimental Plan to accomplish 2020 experimental goals.

# Date

17062020

# Objective

  • To elaborate a realistic plan to continue doing experiments.

# Method

  • Look at the agreed plan from the yearly meeting.
  • Draw scenarios/ possibilities due to currently crisis of covid-19.

# Agreed plan

  1. Measure the essential genes in each of the genetic backgrounds of the trajectory.

# Scenarios to achieve that plan

  • Scenario 1: Ideal

    • Overlap with Enzo and Wessel experiments
      • Enzo and Wessel do the SATAY on $\Delta bem1$ and $\Delta bem1\Delta bem3$
      • Enzo will do the SATAY of $\Delta bem1\Delta bem3\Delta nrp1\Delta bem2$
    • I will do the SATAY and strain construction of $\Delta bem1\Delta bem3\Delta nrp1$
  • Scenario 2: Probable

    • Overlap with Enzo and Wessel experiments
      • Enzo and Wessel do the SATAY on $\Delta bem1$.
      • Enzo will do the SATAY of $\Delta bem1\Delta bem3\Delta nrp1\Delta bem2$
      • I will do the SATAY and strain construction of $\Delta bem1\Delta bem3\Delta nrp1$ and for $\Delta bem1\Delta bem3$.

# Consequences for each scenario

  • Scenario Ideal :

    • Develop a plan for the strain construction (knockout the ade2 gene in ylic128)(~ 2 months). It can start in September 2020.
    • Do the SATAY on all strains (~ ? months)
  • Scenario Probable :

    • Develop a plan to have $\Delta bem1\Delta bem3 $ ura3- ade2-.(~ 3 months) It can start in September 2020.
    • Develop a plan for the strain construction (knockout the ade2 gene in ylic128)(~ 2 months). It can start in September 2020.
    • Do SATAY on all strains.

# Conclusion