# Strain construction database 🐌
| Strain name | Genotype | Nickname | Comments |
|---|---|---|---|
| ByK832 | W303 ura3^0 ade2::NAT | Benoit strain | |
| ByK832-T | W303 ura3^0 ade2::NAT + pBK549 | transformed Benoit strain | |
| ByK352 | W303 ura3^0 ade2::HIS3 | old Benoit strain | |
| ylic128 | W303 , Mating type a bem1::G418 bem3::NAT nrp1::HYGRO ura3^0 | From sporulation of yLL135, not sequenced | |
| ylic130 | W303, Mat type a bem1::G418 bem2::leu2 ura3^0 | From sporulation of yLL135, not sequenced | |
| ylic131 | MAT $\alpha$ technical replicate of yLL3a ($\alpha$) | Liedewij wild type | Sequenced the ADE2 region, see here the DATA.dna |
| ylic132 | MAT $\alpha$ can1-100 leu2-3,112 his3-11,15 BUD4 from S288C ade2::URA3 | clean ade2 delete URA3+ | Transformed from yLL3a, here ALL THE STEPS I have 5 biological replicates of ylic132: . ylic132_1 (ade2 locus sequenced (LINK)) . ylic132_2(ade2 NOT locus sequenced) . ylic132_3 (ade2 locus sequenced) . ylic132_4(ade2 locus sequenced) .ylic132_5(ade2 locus sequenced) |
| ylic133 | MAT $\alpha$ can1-100 leu2-3,112 his3-11,15 BUD4 from S288C ade2 ura3^0 | clean ade2 and ura3 delete | Transformed from ylic132_2 I have 5 biological replicates: - ylic133_1-5 ALL OF THEM ARE SEQUENCED AND THEY ARE CORRECT |
| yEK7a | W303 , Mat a bem1::G418 bem3::NAT nrp1::HYGRO bem2::leu2 , ura3^0 | Enzo full delete | From sporulation of yLL135, not sequenced |
| ylic134 | MAT $\alpha$ can1-100 leu2-3,112 his3-11,15 BUD4 from S288C ade2- URA3 | Transformed with pLL112 plasmid : pRS416 (URA3 CEN6/ARSH4) | From a plasmid transformation to further select for diploids from the mating between yEK7a and ylic134 |
| ylic135 | MAT $\alpha$ can1-100 leu2-3,112 his3-11,15 BUD4 from S288C ade2- ura3- bem2::leu2 | From transformation. Confirmed by colony PCR | So far 2 biological replicates Not yet sequenced |