# Strain construction database 🐌

Strain name Genotype Nickname Comments
ByK832 W303 ura3^0 ade2::NAT Benoit strain
ByK832-T W303 ura3^0 ade2::NAT + pBK549 transformed Benoit strain
ByK352 W303 ura3^0 ade2::HIS3 old Benoit strain
ylic128 W303 , Mating type a
bem1::G418
bem3::NAT nrp1::HYGRO ura3^0
From sporulation of yLL135,
not sequenced
ylic130 W303, Mat type a
bem1::G418
bem2::leu2 ura3^0
From sporulation of yLL135,
not sequenced
ylic131 MAT $\alpha$
technical replicate of yLL3a ($\alpha$)
Liedewij wild type Sequenced the ADE2 region, see here the DATA.dna
ylic132 MAT $\alpha$ can1-100 leu2-3,112 his3-11,15
BUD4 from S288C
ade2::URA3
clean ade2 delete URA3+ Transformed from yLL3a, here ALL THE STEPS
I have 5 biological replicates of ylic132:
. ylic132_1 (ade2 locus sequenced (LINK))
. ylic132_2(ade2 NOT locus sequenced)
. ylic132_3 (ade2 locus sequenced)
. ylic132_4(ade2 locus sequenced)
.ylic132_5(ade2 locus sequenced)
ylic133 MAT $\alpha$ can1-100 leu2-3,112 his3-11,15
BUD4 from S288C
ade2 ura3^0
clean ade2 and ura3 delete Transformed from ylic132_2
I have 5 biological replicates:
- ylic133_1-5 ALL OF THEM ARE
SEQUENCED AND THEY ARE CORRECT
yEK7a W303 , Mat a
bem1::G418
bem3::NAT nrp1::HYGRO bem2::leu2 , ura3^0
Enzo full delete From sporulation of yLL135,
not sequenced
ylic134 MAT $\alpha$ can1-100 leu2-3,112 his3-11,15
BUD4 from S288C
ade2- URA3
Transformed
with pLL112
plasmid : pRS416 (URA3 CEN6/ARSH4)
From a plasmid
transformation to further
select for diploids
from the mating
between yEK7a and ylic134
ylic135 MAT $\alpha$ can1-100 leu2-3,112 his3-11,15
BUD4 from S288C
ade2- ura3- bem2::leu2
From transformation.
Confirmed by
colony PCR
So far 2 biological replicates
Not yet sequenced