1 | Primer 1 pBK549 ADE | 11102018 | 15102018 | gtttcccgactggaaagcg | They worked for the sequencing |
2 | Primer 2 pBK549 ADE | 11102018 | 15102018 | agccccaccagctcc | They worked for the sequencing |
3 | Pirmer 3 pBK549 ADE | 11102018 | 15102018 | acataagaagccatataagtccc | They worked for the sequencing |
4 | Primer 4 pBK549 ADE | 11102018 | 15102018 | atgatcccgtttcgttacc | They worked for the sequencing |
5 | Primer 5 pBK549 ADE | 11102018 | 15102018 | cagctagtttttcgatatcaag | They worked for the sequencing |
6 | Primer 6 pBk549 Tpase | 11102018 | 15102018 | aggaaaaattggcagtaacctg | They worked for the sequencing |
7 | Primer 7 pBK549 Tpase | 11102018 | 15102018 | gtgaaaaggatcatggcaaag | They worked for the sequencing |
8 | Primer 8 pBK549 Tpase | 11102018 | 15102018 | tagtgaatgtgacttggataaatctaaaggg | They worked for the sequencing |
9 | Primer 9 pBK549 Tpase | 11102018 | 15102018 | ttatcatggtggaggggaagg | They worked for the sequencing |
10 | Primer 1 Ade2 | 19102018 | 22102018 | GTATAAATTGGTGCGTAAAATCGTTGGATCTCTCTTCTA | |
11 | Primer 2 Ade2 | 19102018 | 22102018 | TATGTATGAAGTCCACATTTGATGTAATCATAACAAAGCC | |
12 | Primer 3 Ade2 | 24102018 | 25102018 | TAGCGCTATCCTCGGTTCTGCATTG | Primer 200 bp away from primer 1 to test ade2 deletion of ByK832 |
13 | Primer 4 Ade2 | 24102018 | 25102018 | ACACCAACATAACACTGACATCTTTAAC | Primer 200 bp away from primer 2 to test ade2 deletion of ByK832 |
(It did contain an ARS 😦 ) 14 | Upstream forward primer 1 | 28022019 | 1032019 | GATCATTTCGAAAAGTTGCCTAGTTTCATG | To integrate the URA gene into the adenine locus |
15 | Upstream reverse primer 2 | 28022019 | 1032019 | GCTGTGGtatggtgcactctc CTTGATTGTTTTGTCCGATTTTCTTGTTTTTCTTG | They worked for all the PCRs 😃 |
16 | URA forward 3 | 28022019 | 1032019 | CAAGAAAAACAAGAAAATCGGACAAAACAATCAAG gagagtgcaccataCCACAGC | |
17 | URA reverse 4 | 28022019 | 1032019 | GATGTAATCATAACAAAGCCTAAAAAATAGGTATATC GTGAGTTTAGTATACATGCATTTACTTATAATACAG | |
18 | Downstream forward 5 | 28022019 | 1032019 | CTGTATTATAAGTAAATGCATGTATACTAAACTCAC GATATACCTATTTTTTAGGCTTTGTTATGATTACATC | |
19 | Downstream reverse 6 | 28022019 | 1032019 | GGTGTTAAGAGTACTGAGTGAACATATAGAAAAGG | |
20 | Upstream URA removal 7 | 14032019 | 26032019 | GATGTAATCATAACAAAGCCTAAAAAATAGGTATATCCTTGATTGTTTTGTCCGATTTTCTTGTTTTTCTTG | To make the construct to be inserted when removing the URA |
21 | Downstream URA removal 8 | 14032019 | 26032019 | CAAGAAAAACAAGAAAATCGGACAAAACAATCAAGGATATACCTATTTTTTAGGCTTTGTTATGATTACATC | |
22 | Primer 9 ADE Forward Check | 29042019 | 30042019 | GAAAGCTTTTGACCAGGTTATTATAAAAGAAACTTC | To check the insertion of the 1st Transformation , to insert the URA on the ADE locus |
23 | Primer 10 Reverse Check | 29042019 | 30042019 | CATATTGGAAGACCTTCCAAGGGAACATTATAG | |
24 | Primer 1_new Upstream forward | 30042019 | 6052019 | ATTACAGCTATGCTGACAAATGACTCTTG | In replace of primer 1 upstream forward (GATCATTTCGAAAAGTTGCCTAGTTTCATG) after the Ars region |
25 | Primer 5_downstream_forward_new | 21052019 | 23052019 | CTGTATTATAAGTAAATGCATGTATACTAAACTCACTATATAAGTTTATTGATATACTTGTACAGCAAATAATTATAAAA | This primer does not work with primer 4 ura reverse,In replace of primer 5 downstream forward to be just next to ADe2 gene , at the cost of having less GC content and longer primer |
26 | Primer 6_downstream_reverse_NO_RGA1 | 21052019 | 23052019 | GCTATCCTCGGTTCTGCATTGAGC | In replace of primer 6 downstream reverse to be outside the RGA1 region |
| Primer 4_new_ura_reverse | | | TTTTATAATTATTTGCTGTACAAGTATATCAATAAACTTATATAGTGAGTTTAGTATACATGCATTTACTTATAATACAG | to have an overlap with primer 5 _new_downstream_forward |
27 | Primer1-nrp1-upstream | 10022020 | 11022020 | GAAGACAGTGAGTAGGCG//CGATGACGAAGACGATGAAGACA | To delete nrp1 with the HyGRO cassette -it did not work for yll137 , however the other primer M83/84 and SPY1 and SPY2 did work, from Els! |
28 | Primer2-nrp1-upstream | 10022020 | 11022020 | CTATAGTGTCACCTAAATCGTATGTG TAGCAATGCACAATTATCCTAGCGC | |
29 | Primer3-hygro-upstream | 10022020 | 11022020 | GCGCTAGGATAATTGTGCATTGCTACACATACGATTTAGGTGACACTATAG | |
30 | Primer4-hygro-downstream | 10022020 | 11022020 | GACCTCGCCTGTTCCTAACGAAATTAATACGACTCACTATAGGGAGACC | |
31 | Primer5-nrp1-downstream | 10022020 | 11022020 | GGTCTCCCTATAGTGAGTCGTATTAATTTCGTTAGGAACAGGCGAGGTC | |
32 | Primer6-nrp1-downstream | 10022020 | 11022020 | GCTTAAGAACCGTCTTGAAGTCTGATG// CTGCCGCTGGTGAAGAAATTTC | To delete nrp1 with the HyGRO cassette -it did not work for yll137 , however the other primer oES84 did work, from Els! |
33 | Primer1-bem2-upstream | 10022020 | 11022020 | CTACGTTGCAGCCACTGGTAC | To delete bem2 with the LEU2 marker// did work for yll140! |
34 | Primer2-bem2-upstream | 10022020 | 11022020 | gatagcgcccctgtgtgttcGTGTCTATCCAGAAAAGGCACGAC | |
35 | Primer3-leu2-upstream | 10022020 | 11022020 | GTCGTGCCTTTTCTGGATAGACACgaacacacaggggcgctatc | |
36 | Primer4-leu2-downstream | 10022020 | 11022020 | CTCTCTCAGCAGTGGATTGTATACcctccaatatcaaattaggaatcgtagtttcatg | |
37 | Primer 5-bem2-downstream | 10022020 | 11022020 | catgaaactacgattcctaatttgatattggaggGTATACAATCCACTGCTGAGAGAG | |
38 | Primer 6-bem2-downstream | 10022020 | 11022020 | CAGGCGGAAAGAAGGCAATTG | |
39 | SPY3-nrp1 | 28022020 | 3032020 | GGGAAATAGTATTGTCGATTGGCATG | To check the insertion of the hygro in the nrp1 locus |
40 | SPY4-nrp1 | 28022020 | 3032020 | CCAAGATCATTGCCATTGACATTAAC | To check the insertion of the hygro in the nrp1 locus |
41 | SPY1-bem2 | 28022020 | 3032020 | GAGAACACAAGATATCAGACGGC | To check the insertion of the leu2 marker in the bem2 locus |
42 | SPY2-bem2 | 28022020 | 3032020 | CTTATCGTCCGCTGTGGTCC | |
43 | hygro-forward | 3032020 | 4032020 | GCCTGACCTATTGCATCTCCC | To check from inside the hygro or the nrp1 gene into the clones and controls |
44 | hygro-reverse | 3032020 | 4032020 | CTCGCTGAATTCCCCAATGTC | |
45 | nrp1-forward | 3032020 | 4032020 | GGTTTACCCAATATGGTGTTAGACCAG | |
46 | nrp1-reverse | 3032020 | 4032020 | GATTCCAACTCACTTTGAGTTGTGTCG | |
47 | bem2-out-upstream | 24032020 | | TCTAGAATACGCATCATTACGGAGATTCTG | To check inside bem2 and leu2 markers for correct integration |
48 | bem2-out-downstream | 24032020 | | CGGCTAGTTCTAGTGACCTCAC | |
49 | leu2-out-upstream | 240302020 | | ctggaacggtgtattgttcactatcc | |
50 | leu-out-downstream | 24032020 | | ggccctacaacatgagccac | |