Experimental-Journal-PhD

# Primer database 🐒

Primer Number Primer name Order date Receiving date Sequence Comments
1 Primer 1 pBK549 ADE 11102018 15102018 gtttcccgactggaaagcg They worked for the sequencing
2 Primer 2 pBK549 ADE 11102018 15102018 agccccaccagctcc They worked for the sequencing
3 Pirmer 3 pBK549 ADE 11102018 15102018 acataagaagccatataagtccc They worked for the sequencing
4 Primer 4 pBK549 ADE 11102018 15102018 atgatcccgtttcgttacc They worked for the sequencing
5 Primer 5 pBK549 ADE 11102018 15102018 cagctagtttttcgatatcaag They worked for the sequencing
6 Primer 6 pBk549 Tpase 11102018 15102018 aggaaaaattggcagtaacctg They worked for the sequencing
7 Primer 7 pBK549 Tpase 11102018 15102018 gtgaaaaggatcatggcaaag They worked for the sequencing
8 Primer 8 pBK549 Tpase 11102018 15102018 tagtgaatgtgacttggataaatctaaaggg They worked for the sequencing
9 Primer 9 pBK549 Tpase 11102018 15102018 ttatcatggtggaggggaagg They worked for the sequencing
10 Primer 1 Ade2 19102018 22102018 GTATAAATTGGTGCGTAAAATCGTTGGATCTCTCTTCTA
11 Primer 2 Ade2 19102018 22102018 TATGTATGAAGTCCACATTTGATGTAATCATAACAAAGCC
12 Primer 3 Ade2 24102018 25102018 TAGCGCTATCCTCGGTTCTGCATTG Primer 200 bp away from primer 1 to test ade2 deletion of ByK832
13 Primer 4 Ade2 24102018 25102018 ACACCAACATAACACTGACATCTTTAAC Primer 200 bp away from primer 2 to test ade2 deletion of ByK832
(It did contain an ARS 😦 ) 14 Upstream forward primer 1 28022019 1032019 GATCATTTCGAAAAGTTGCCTAGTTTCATG To integrate the URA gene into the adenine locus
15 Upstream reverse primer 2 28022019 1032019 GCTGTGGtatggtgcactctc CTTGATTGTTTTGTCCGATTTTCTTGTTTTTCTTG They worked for all the PCRs 😃
16 URA forward 3 28022019 1032019 CAAGAAAAACAAGAAAATCGGACAAAACAATCAAG gagagtgcaccataCCACAGC
17 URA reverse 4 28022019 1032019 GATGTAATCATAACAAAGCCTAAAAAATAGGTATATC GTGAGTTTAGTATACATGCATTTACTTATAATACAG
18 Downstream forward 5 28022019 1032019 CTGTATTATAAGTAAATGCATGTATACTAAACTCAC GATATACCTATTTTTTAGGCTTTGTTATGATTACATC
19 Downstream reverse 6 28022019 1032019 GGTGTTAAGAGTACTGAGTGAACATATAGAAAAGG
20 Upstream URA removal 7 14032019 26032019 GATGTAATCATAACAAAGCCTAAAAAATAGGTATATCCTTGATTGTTTTGTCCGATTTTCTTGTTTTTCTTG To make the construct to be inserted when removing the URA
21 Downstream URA removal 8 14032019 26032019 CAAGAAAAACAAGAAAATCGGACAAAACAATCAAGGATATACCTATTTTTTAGGCTTTGTTATGATTACATC
22 Primer 9 ADE Forward Check 29042019 30042019 GAAAGCTTTTGACCAGGTTATTATAAAAGAAACTTC To check the insertion of the 1st Transformation , to insert the URA on the ADE locus
23 Primer 10 Reverse Check 29042019 30042019 CATATTGGAAGACCTTCCAAGGGAACATTATAG
24 Primer 1_new Upstream forward 30042019 6052019 ATTACAGCTATGCTGACAAATGACTCTTG In replace of primer 1 upstream forward (GATCATTTCGAAAAGTTGCCTAGTTTCATG) after the Ars region
25 Primer 5_downstream_forward_new 21052019 23052019 CTGTATTATAAGTAAATGCATGTATACTAAACTCACTATATAAGTTTATTGATATACTTGTACAGCAAATAATTATAAAA This primer does not work with primer 4 ura reverse,In replace of primer 5 downstream forward to be just next to ADe2 gene , at the cost of having less GC content and longer primer
26 Primer 6_downstream_reverse_NO_RGA1 21052019 23052019 GCTATCCTCGGTTCTGCATTGAGC In replace of primer 6 downstream reverse to be outside the RGA1 region
Primer 4_new_ura_reverse TTTTATAATTATTTGCTGTACAAGTATATCAATAAACTTATATAGTGAGTTTAGTATACATGCATTTACTTATAATACAG to have an overlap with primer 5 _new_downstream_forward
27 Primer1-nrp1-upstream 10022020 11022020 GAAGACAGTGAGTAGGCG//CGATGACGAAGACGATGAAGACA To delete nrp1 with the HyGRO cassette -it did not work for yll137 , however the other primer M83/84 and SPY1 and SPY2 did work, from Els!
28 Primer2-nrp1-upstream 10022020 11022020 CTATAGTGTCACCTAAATCGTATGTG TAGCAATGCACAATTATCCTAGCGC
29 Primer3-hygro-upstream 10022020 11022020 GCGCTAGGATAATTGTGCATTGCTACACATACGATTTAGGTGACACTATAG
30 Primer4-hygro-downstream 10022020 11022020 GACCTCGCCTGTTCCTAACGAAATTAATACGACTCACTATAGGGAGACC
31 Primer5-nrp1-downstream 10022020 11022020 GGTCTCCCTATAGTGAGTCGTATTAATTTCGTTAGGAACAGGCGAGGTC
32 Primer6-nrp1-downstream 10022020 11022020 GCTTAAGAACCGTCTTGAAGTCTGATG// CTGCCGCTGGTGAAGAAATTTC To delete nrp1 with the HyGRO cassette -it did not work for yll137 , however the other primer oES84 did work, from Els!
33 Primer1-bem2-upstream 10022020 11022020 CTACGTTGCAGCCACTGGTAC To delete bem2 with the LEU2 marker// did work for yll140!
34 Primer2-bem2-upstream 10022020 11022020 gatagcgcccctgtgtgttcGTGTCTATCCAGAAAAGGCACGAC
35 Primer3-leu2-upstream 10022020 11022020 GTCGTGCCTTTTCTGGATAGACACgaacacacaggggcgctatc
36 Primer4-leu2-downstream 10022020 11022020 CTCTCTCAGCAGTGGATTGTATACcctccaatatcaaattaggaatcgtagtttcatg
37 Primer 5-bem2-downstream 10022020 11022020 catgaaactacgattcctaatttgatattggaggGTATACAATCCACTGCTGAGAGAG
38 Primer 6-bem2-downstream 10022020 11022020 CAGGCGGAAAGAAGGCAATTG
39 SPY3-nrp1 28022020 3032020 GGGAAATAGTATTGTCGATTGGCATG To check the insertion of the hygro in the nrp1 locus
40 SPY4-nrp1 28022020 3032020 CCAAGATCATTGCCATTGACATTAAC To check the insertion of the hygro in the nrp1 locus
41 SPY1-bem2 28022020 3032020 GAGAACACAAGATATCAGACGGC To check the insertion of the leu2 marker in the bem2 locus
42 SPY2-bem2 28022020 3032020 CTTATCGTCCGCTGTGGTCC
43 hygro-forward 3032020 4032020 GCCTGACCTATTGCATCTCCC To check from inside the hygro or the nrp1 gene into the clones and controls
44 hygro-reverse 3032020 4032020 CTCGCTGAATTCCCCAATGTC
45 nrp1-forward 3032020 4032020 GGTTTACCCAATATGGTGTTAGACCAG
46 nrp1-reverse 3032020 4032020 GATTCCAACTCACTTTGAGTTGTGTCG
47 bem2-out-upstream 24032020 TCTAGAATACGCATCATTACGGAGATTCTG To check inside bem2 and leu2 markers for correct integration
48 bem2-out-downstream 24032020 CGGCTAGTTCTAGTGACCTCAC
49 leu2-out-upstream 240302020 ctggaacggtgtattgttcactatcc
50 leu-out-downstream 24032020 ggccctacaacatgagccac

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