# Title: 22072019-Sequencing of ylic133 biological replicates 🆗 ✅

# Date

22072019

# Objective

To finally check that neither the adenine , neither ura is in the genome of these cells

# Method

• 10ul Final sample prepration
• 250ng purified PCR product
• 2,5 ul primer 22
• 2,5 ul primer 23
• Rest with MiliQ
• Send for sequencing the clones 1-3 and 7-9

# Results 😃

• The results shows a complete coverage of the DNA without URA and ADE2
• With the DNA with URA , we can see that we dont have any URA contained in any of the ylic133 biological replicates. 😃

# Conclusion

Everything did work! So, now I can continue with the crossing of the strains with bem1 deleted, bem1bem3d, bem1bem3dnrp1d, and dbem1dbem3dnrp1dbem2.