# Title: 22072019-Sequencing of ylic133 biological replicates 🆗 ✅

# Date

22072019

# Objective

To finally check that neither the adenine , neither ura is in the genome of these cells

# Method

  • 10ul Final sample prepration
  • 250ng purified PCR product
  • 2,5 ul primer 22
  • 2,5 ul primer 23
  • Rest with MiliQ
  • Send for sequencing the clones 1-3 and 7-9

# Results 😃

  • The results shows a complete coverage of the DNA without URA and ADE2
  • With the DNA with URA , we can see that we dont have any URA contained in any of the ylic133 biological replicates. 😃

# Conclusion

Everything did work! So, now I can continue with the crossing of the strains with bem1 deleted, bem1bem3d, bem1bem3dnrp1d, and dbem1dbem3dnrp1dbem2.