# Title : 24072019-Strategies to generate the strains I want to study using SATAY 📃

# Date

Wednesday 24072019

# Objective

To assess what is the best strategy to go further in terms , of realiability and background compatibility.

# Method

Strategy Pros Cons
Strategy 1 - Cross yEK7a: dbem1dbem3dnrp1dbem2 ura3
(from Enzo) with ylic133 (alpha)
- Sporulate the diploid and select
for all deletions without ura3 and ade2.
- Cross again with ylic133 and have
homozygous ura3 and ade2 auxotrophies.
I keep the same background as ylic133
After having the homozygous diploid
I just select for the different
target genes.
Could be work demanding
Strategy 2 - Cross ylic128(dbem1dbem3dnrp1) with ylic133
to form a diploid, homozygous in ura3-
- Sporulate and select for
dbem1dbem3dnrp1 ura3- ade2-
Cross againg with ylic133 to have
Same as Strategy 1, just I wont have
bem2 in the study
- Work demanding,
because I have to select
for many markers

# Results

# Conclusion