# Title: 31072019 Double check Byk832T biolog. replicates suitability for SATAY.
# Date
Wednesday, 31072019
# Objective
To be sure that the strains we are using, which are the Byk832 + pBK549, are the correct one to start the SATAY.
Essentially , they have to fulfill two main requirements:
- Full growth in SD-URA
- Few growth in SD-ADE
# Method
- Re-streaking from the glycerol stocks of each of the strains onto -ura and - ade plates.
# Results
1st line of plates : -URA plates
2nd line of plates: -ade plates
# Conclusion
- We can observe prominent growth in the -ade plates, indicating our transformed strains are not the ideal strains to continue with the SATAY.
- We should transform them again with pBK549 and also transform another strain like yll3a with that plasmid , and compare both of them.