92. Title: 26062019-Sequencing of the URA integration in ade2 locus in ylic132 :white_check_mark:

92.1. Date:

26062019

92.2. Objective:

To check if the transformation with the URA3+ promoter with ade2 flanking regions was indeed what we expect according the selective growth on -URA plates and the pink phenotype of lacking the adenine.

92.3. Method

I use primers 24 and primer 26 to do the PCR on the genomic DNA of ylic132 to send for sequencing.

primer 24: ATTACAGCTATGCTGACAAATGACTCTTG

primer 26: GCTATCCTCGGTTCTGCATTGAGC

92.4. Results

92.5. Conclusion

• point mutation of a ‘C’ instead of a ‘T’ in the ura promoter, however, it does not seem to cause any affect.

• I will do sequencing of 7 more biological replicates of ylic132 to compare with this one.