Title : how do yeast cells look like during log phase at different galactose concentrations? (2nd experiment)
Contents
6. Title : how do yeast cells look like during log phase at different galactose concentrations? (2nd experiment)#
6.1. Date#
07112022 (Monday)- 11112022 (Thursday)
6.2. Objective#
Take images with a proper density because the previous experiment with the original densities was too crowded for the WT strain.
Show that during log phase yeast cells with the gal promoter look OK at different galactose concentrations.
Genotypes:
WT+sfGFP+pGal-CDC42(ywkd065a)
dbem1+sfGFP+pGal-CDC42(ywkd069b)
dbem1dbem3+sfGFP+pGal-CDC42(ywkd070a)
WT(yll3a Control)
Gal concentrations:
0%, 0.01%,0.02%,0.05%,0.06%,0.08%,0.1%,0.2%,2%
6.3. Method#
Population growth measurements in 0,0.01,0.02,0.05,0.06,0.08,0.1,0.2,2% galactose for the following strains: WT+sfGFP+pGal-CDC42(ywkd065a),dbem1+sfGFP+pGal-CDC42(ywkd069b),dbem1dbem3+sfGFP+pGal-CDC42(ywkd070a),WT(yll3a Control).
In the log phase of the measuring part take some bright field images of the cell morphology in the 96 well plate.
I transfer 50ul of cells to an 96 well imaging plate to take the bright field images.
Dilution of cells:
WT: all galactose concentrations: 1:1000
WT+pGal : more than 0.02% gal: 1:100
dbem1+pGal : more than 0.06% gal: 1:100
dbem1dbem3+pGal : more than 0.05% gal: 1:100