Title : “Biotek experiment for new dbem1 pGal-cdc42 strain”
Contents
19. Title : “Biotek experiment for new dbem1 pGal-cdc42 strain”#
19.1. Date#
26042021-30042021
19.2. Objective#
To check the phenotype and the population growth behaviour of the new dbem1 +pGal-cdc42 strain.
19.3. Method#
1ul of glycerol stock in 100ul media
incubation in 30C in the biotek
Media base: 4xCSM-NF+2%Raff
Plate layout:
200x dilution from incubation to measuring : 0.5ul cells in 100ul media.
19.4. Results#
19.5. Conclusion#
Notice that the new dbem1 strain do not grow in 0% Gal after 48hours of dilution in 0% Gal.
The dbem1dbem3 strain seems very slow.