27. Samples

27.1. Ligated DNAs

Strain	Amount	Number of ligations
wild type (WT)	200uL	2X=20PCRs for sequencing
dnrp1(1)	200uL	2X=20PCRs for sequencing
dnrp1(2)	200uL	2X=20PCRs for sequencing

27.2. PCR test on gel for each ligation

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27.3. Genomic DNAs

Strain	Amount	Concentration	Volume
wild type (WT)	~50ug	65ng/uL	~760uL
dnrp1(1)	~40ug	44ng/uL	~900uL
dnrp1(2)	~32ug	26ng/uL	~1200uL

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