80. Title: 22072019-Sequencing of ylic133 biological replicates :ok: :white_check_mark:#

80.1. Date#

22072019

80.2. Objective#

To finally check that neither the adenine , neither ura is in the genome of these cells

80.3. Method#

  • 10ul Final sample prepration

  • 250ng purified PCR product

  • 2,5 ul primer 22

  • 2,5 ul primer 23

  • Rest with MiliQ

  • Send for sequencing the clones 1-3 and 7-9

80.4. Results :)#

  • The results shows a complete coverage of the DNA without URA and ADE2

  • With the DNA with URA , we can see that we dont have any URA contained in any of the ylic133 biological replicates. :)

80.5. Conclusion#

Everything did work! So, now I can continue with the crossing of the strains with bem1 deleted, bem1bem3d, bem1bem3dnrp1d, and dbem1dbem3dnrp1dbem2.