12. Title : Biotek volume reduced during incubation at 30C to check supressors#

12.1. Date#

28092021-03102021

12.2. Objective#

  • To see if the raise of suppresors is prevented if the volume of the well is reduced , and also the number of cells to start with are increased.

12.3. Method#

  • 10 ul of glycerol stock in 30 ul media

  • incubation in 30C in the biotek

  • base media: 4xCSM-LF+2% Raffinose

Note: With 30uL media still the bottom of the weel is not fully covered by the media. This affect the OD measurements because there are empty spots in the well.

12.3.1. For measuring#

  • 1ul from incubation plate in 100ul media

  • incubation in 30C in the biotek

  • base media: 4xCSM-LF+2% Raffinose

12.4. Results#

End of incubation

  • We can see weird curve shapes for the control rows which are given to the low measurement volume and not by contamination . The wells were checked afterwards in the media for transparent.

End of measuring

End of measuring II

Observation: The well B9 was accidently without any liquid so the line is shown is an artefact is not real data.

12.5. Conclusion#