Title: Transformation on nrp1::HYGRO with bem1::KANMX
Contents
21. Title: Transformation on nrp1::HYGRO with bem1::KANMX#
21.1. Date#
21.2. Objective#
21.3. Method#
gDNA from yll117 : dbem1::KANMX dbem3::NAT
primers : olic54/55
PCR with gDNA from yll117 with olic54 and olic55 primers
PCR product purified : 200ng/uL (>30uL left from dbem1dbem3 transformation)