20. Title : Genomic extraction and PCR of clean ylic137_2#

20.1. Date#

01032021-03032021

20.2. Objective#

  • To check that the population of cells from ylic137_2 is purely having the casette bem3::NAT and not a mixed population as we saw in here.

20.3. Method#

  • gDNA extraction

  • 12mL cells after overnight incubation

  • 2ml culture per eppi

  • 30ul elution

  • gDNA concentration = 52 ng/uL quantified in gel

  • PCR at 65C annealing temperature

    • 1ul gDNA

  • 03032021: Temperature gradient PCR to find the optimum PCR conditions

20.4. Results#

20.5. Conclusion#

  • Successfully clean ylic137_2 stock