66. Title : 24092019-Mating of ylic134 with yEK7a :broken_heart:#

66.1. Date#

24092019-26092019

66.2. Objective#

To get a diploid that I am able to select for with marker selection.

66.3. Method#

  • 23092019: Incubation of single colonies and not multiple colonies (from the transformation plate) of ylic134 in CSM-URA , and yEk7a from glycerol stock.

  • 24092019: Clear tubes :( , no growth of the colonies taken of ylic134 (maybe it is because ylic133_3 still grows very slowly)

    • I took more colonies from the transformation plate and transfer them grow in CSM-URA

    • I did replica plating of the transformation plate onto a another -ura plate , to check the viability of the transformants on -ura.

66.4. Results#

  • The transformation with pLL112 plasmid did not work.

  • No colonies did grow in the replica plate in -URA

66.5. Conclusion#

  • The mating never could happened because I did not get any ylic133+pLL112 cells :(