Strain construction database :snail:

Strain name	Genotype	Nickname	Comments
ByK832	W303 ura3^0 ade2::NAT	Benoit strain
ByK832-T	W303 ura3^0 ade2::NAT + pBK549	transformed Benoit strain
ByK352	W303 ura3^0 ade2::HIS3	old Benoit strain
ylic128	W303 , Mating type a bem1::G418 bem3::NAT nrp1::HYGRO ura3^0		From sporulation of yLL135, not sequenced
ylic130	W303, Mat type a bem1::G418 bem2::leu2 ura3^0		From sporulation of yLL135, not sequenced
ylic131	MAT \(\alpha\) technical replicate of yLL3a (\(\alpha\))	Liedewij wild type	Sequenced the ADE2 region, see here the DATA.dna
ylic132	MAT \(\alpha\) can1-100 leu2-3,112 his3-11,15 BUD4 from S288C ade2::URA3	clean ade2 delete URA3+	Transformed from yLL3a, here ALL THE STEPS I have 5 biological replicates of ylic132: . ylic132_1 (ade2 locus sequenced (LINK)) . ylic132_2(ade2 NOT locus sequenced) . ylic132_3 (ade2 locus sequenced) . ylic132_4(ade2 locus sequenced) .ylic132_5(ade2 locus sequenced)
ylic133	MAT \(\alpha\) can1-100 leu2-3,112 his3-11,15 BUD4 from S288C ade2 ura3^0	clean ade2 and ura3 delete	Transformed from ylic132_2 I have 5 biological replicates: - ylic133_1-5 ALL OF THEM ARE SEQUENCED AND THEY ARE CORRECT
yEK7a	W303 , Mat a bem1::G418 bem3::NAT nrp1::HYGRO bem2::leu2 , ura3^0	Enzo full delete	From sporulation of yLL135, not sequenced
ylic134	MAT \(\alpha\) can1-100 leu2-3,112 his3-11,15 BUD4 from S288C ade2- URA3	Transformed with pLL112 plasmid : pRS416 (URA3 CEN6/ARSH4)	From a plasmid transformation to further select for diploids from the mating between yEK7a and ylic134
ylic135	MAT \(\alpha\) can1-100 leu2-3,112 his3-11,15 BUD4 from S288C ade2- ura3- bem2::leu2	From transformation. Confirmed by colony PCR	So far 2 biological replicates Not yet sequenced
ylic136	MAT \(\alpha\) can1-100 leu2-3,112 his3-11,15 BUD4 from S288C ade2- ura3- nrp1::HYGRO	From transformation. Confirmed by colony PCR	So far 4 biological replicates Not yet sequenced