Title: 31072019 Double check Byk832T biolog. replicates suitability for SATAY.
Contents
75. Title: 31072019 Double check Byk832T biolog. replicates suitability for SATAY.#
75.1. Date#
Wednesday, 31072019
75.2. Objective#
To be sure that the strains we are using, which are the Byk832 + pBK549, are the correct one to start the SATAY.
Essentially , they have to fulfill two main requirements:
Full growth in SD-URA
Few growth in SD-ADE
75.3. Method#
Re-streaking from the glycerol stocks of each of the strains onto -ura and - ade plates.
75.4. Results#
1st line of plates : -URA plates
2nd line of plates: -ade plates
75.5. Conclusion#
We can observe prominent growth in the -ade plates, indicating our transformed strains are not the ideal strains to continue with the SATAY.
We should transform them again with pBK549 and also transform another strain like yll3a with that plasmid , and compare both of them.