16. Title: Biotek 30C with new Gal concentration below 0.1%#

16.1. Date#

30082021-18092021

16.2. Objective#

  • To measure all gal strains in 30C, and to measure a gal concentration below 0,1%

16.3. Method#

  • 1ul of glycerol stock in 100ul media

  • incubation in 30C in the biotek

  • Media base: 4xCSM-NF+2%Raff

  • Gal concentrations: 0,0.002,0.01,0.06,0.1

  • Plate layout:

  • Measuring :

    • 1ul of grown culture to 100ul media

    • Measuring at 30C

16.4. Results#

Growth in 0% Gal :(

Overnight growth in 0% and 0.002%

  • It is confusing that , for example for the WT strain with the gal promoter there is growth at 0% but very little and some times none in 0.002%.

16.4.1. Repeat the experiment with new fresh media#

  • Make new media 4xCSM-LF+2% Raffinose

  • 1ul of glycerol stock in 100ul media

  • incubation in 30C in the biotek

End of incubation

End of measuring