18. Title: Biotek - 1st experiment for paper all strains#

18.1. Date#

06052021-10052021

18.2. Objective#

  • To check that the sfGFP do not influence the phenotype of the population growth of the pGal strains

18.3. Method#

  • Population growth rate in the Biotek

  • Layout:Two plates

  • Incubation at 11:15

  • Initial amount from thawed glycerol stock:

    • 5ul for ywkd073I and ywkd073II (from previous miserable growth)

    • 1ul for the rest

  • Measuring 08052021

    • 200x dilution for all strains

18.4. Results#

18.4.1. After incubation#

18.4.2. After measuring#

  • Observations

    • ywkd073 (dbem1dbem3) during the measuring part grows extremely miserable

      • perhaps next time do not dilute 200x but 100x

    • all gal strains shows the expected behaviour at 0% gal. (with the exception of ywkd071II and ywkd073II in plate 2)

    • ylic139 (dbem1 pgal) shows growth already in 0.01% gal in both plates while ywkd069a (dbem1 pgal-sfGFP) in 0.05% gal.

18.5. Conclusion#

  • Data analysis:

  • Next experiment on 17052021