Title: 22082019 Mating using liquid drops of yEK7a and ylic133_5 :broken_heart:

70. Title: 22082019 Mating using liquid drops of yEK7a and ylic133_5 :broken_heart:#

70.1. Date#

22082019

Goal: To try to achieve a more efficient mating between ylic133 and yEK7a, because the previous attempt did not get good results after sporulation.

Grow an overnight culture of each of the strains in 10mL YPD
Next morning take a YPD agar plate and add 20ul of each dense liquid culture one on top of each other.
Microscopy time lapse imaging of the mixture of both strains 100ul of 150X yEK7a (highly dense) and ylic133_5 (no dense at all) no diluted , after being washed in non fluorescence media (CSM).
23082019-Restreaking of the mated patch onto a YPD+G418+NAT+Hygro plate.
26082019-Inoculation of 8 restreaked cells to sporulation next morning.

There are “weird structures” on the ylic133_5 well, flowers like structures. It could be some sort of contamination, but which type?
Jut one cell appear to show this dumbell shape (top left last frames) :(
As a next step , I will image all my glycerol stocks from ylic133 and ylic132
ylic133
ylic132

I dont see these weird structures in the Images of my stock meaning that what I saw during the mating was maybe something acquired outside the glycerol stock.