41. Date#

17062020

42. Objective#

  • To elaborate a realistic plan to continue doing experiments.

43. Method#

  • Look at the agreed plan from the yearly meeting.

  • Draw scenarios/ possibilities due to currently crisis of covid-19.

43.1. Agreed plan#

  1. Measure the essential genes in each of the genetic backgrounds of the trajectory.

43.2. Scenarios to achieve that plan#

  • Scenario 1: Ideal

    • Overlap with Enzo and Wessel experiments

      • Enzo and Wessel do the SATAY on \(\Delta bem1\) and \(\Delta bem1\Delta bem3\)

      • Enzo will do the SATAY of \(\Delta bem1\Delta bem3\Delta nrp1\Delta bem2\)

    • I will do the SATAY and strain construction of \(\Delta bem1\Delta bem3\Delta nrp1\)

  • Scenario 2: Probable

    • Overlap with Enzo and Wessel experiments

      • Enzo and Wessel do the SATAY on \(\Delta bem1\).

      • Enzo will do the SATAY of \(\Delta bem1\Delta bem3\Delta nrp1\Delta bem2\)

    • I will do the SATAY and strain construction of \(\Delta bem1\Delta bem3\Delta nrp1\) and for \(\Delta bem1\Delta bem3\).

43.3. Consequences for each scenario#

  • Scenario Ideal :

    • Strain construction for \(\Delta bem1\Delta bem3\Delta nrp1\) (knockout the ade2 gene in ylic128)(~ 2 months). It can start in September 2020.

    • Do the SATAY on this strain (~ ? months)

  • Scenario Probable :

    • Develop a plan to have \(\Delta bem1\Delta bem3\) ura3- ade2-.(~ 3 months) It can start in September 2020.

    • Strain construction for \(\Delta bem1\Delta bem3\Delta nrp1\) (knockout the ade2 gene in ylic128)(~ 2 months). It can start in September 2020.

    • Do SATAY on \(\Delta bem1\Delta bem3\) and \(\Delta bem1\Delta bem3\Delta nrp1\).

44. Conclusion#

  • The constant in the plan is the strain construction and the SATAY on \(\Delta bem1\Delta bem3\Delta nrp1\).